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. Peak Shape 2 Retention Variation 3 Ghost Peaks 4 Column Backpressure 5 Introduction 6 1.
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  • Peak fronting or tailing may occur due to poor quality of the HPLC column or dead volume of the system.
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    Popular replies (1) The quick answer is, the reason is poor method quality due to lack of general training.

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    The peak in (b) is an example of fronting, which most often is the result of overloading the column with sample.

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  • Table I LC troubleshooting tips for improving peak shape.
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  • peak width x 1 mLmin) giving a maximum indicated injection volume of 15l.
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